Sepsis Genomics Pacbio 25788

ARP Genomics Pacbio Raw Data: Streptococcus pneumoniae 180/2 THY broth Replicate 1

Data and Resources

Please see the initiative Data Policy for attribution information.

Field	Value
Resource Permissions	organization_member
analytical_facility	Ramaciotti
archive_ingestion_date	2016-09-12
cell_position	B01
contact_researcher	James Paton/Claudia Trappetti
contextual_data_submission_date	2016-04-26
culture_collection_date	1989-07-28
data_generated	True
data_type	Main dataset
experimental_replicate	1
experimental_sample_preparation_method	Qiagen kit with 100/G genomic tip
facility	RAMACIOTTI
gram_stain	POS
growth_condition_media	THY broth
growth_condition_notes	THY broth (recommend using filtered not autoclaved broth as some of the strains don't seem to grow when media has been overheated). If pelleting, serotype 3 forms large loose pellets compared to other pneumococci due to slimy capsule so they may need to be spun longer. Can be difficult to remove all media, therefore PBS wash is sometimes carried out.
growth_condition_temperature	37.0
growth_condition_time_(h)	No shaking, grow daytime
growth_media	THY broth
host_location	South Australia, Australia (Adelaide)
insert_size_range	15-50kb
isolation_source	Ear
key_virulence_genes	Unknown
library_construction_protocol	20kb template prep_blue pippin
omics	Genomics
publication_reference	Infect Immun. 81(2):505-13 (2013)
replicate	1
rs_version	RS Remote v2.3
sample_id	102.100.100/25788
sample_submission_date	2016-06-07
sequencer	PacBio RSII
sequencer_run_id	m160815_112904_42272
serovar	3
smrt_cell_id	c101035772550000001823252711171631
strain_description	ST180
strain_or_isolate	180/2
taxon_or_organism	Streptococcus pneumoniae
ticket	BPAOPS-2
work_order	Work order 3