ARP Proteomics MS1Quantification 51253
Dataset size is: 3.44 GiB
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Additional Info Show Blank Fields
| Field | Value |
|---|---|
| Resource Permissions | organization_member |
| acquisition_mode_fragmentation | DDA Top12 / HCD |
| analytical_platform | MS1 quantification |
| base_url | https://downloads-qcif.bioplatforms.com/bpa/sepsis/proteomics/raw/ms1quantification/mbpf/BPAOPS-665/ |
| contact_researcher | Mark Walker |
| contextual_data_submission_date | 2018-04-05 |
| culture_collection_date | pre-2000 |
| culture_collection_id | SP137 |
| data_generated | True |
| data_type | Main dataset |
| facility | MBPF |
| facility_code | mbpf |
| gradient_time | 120.0 |
| gram_stain | POS |
| growth_condition_growth_phase | N/A |
| growth_condition_media | Pooled sera |
| growth_condition_od600_reading | N/A |
| growth_condition_temperature | 37.0 |
| growth_condition_time_(h) | 2 hrs exposure to sera |
| growth_media | Pooled sera |
| host_age | NA |
| host_description | NA |
| host_disease_outcome | Toxic shock syndrome and necrotizing fasciitis |
| host_dob | NA |
| host_location | Ontario, Canada |
| isolation_source | Blood |
| key_virulence_genes | SpeB +ve |
| lc_column_type | Thermo Ultimate 3000 RSLCnano / Thermo Acclaim PepMap RSLC P/N 164540 |
| mass_spectrometer | Thermo QExactivePlus |
| omics | Proteomics |
| peptide_resuspension_protocol | Tip-probe sonicate 2x 30s in solvent intended for injection (e.g 90% MeCN/0.1% TFA) |
| publication_reference | Infect Immun 68: 6362-6369 (2000) |
| raw_file_name | 51253_SEP_MBPF_MS_QEPlus1_P16_0062_Exp17.raw |
| replicate | 6 |
| sample_id | 102.100.100/51253 |
| sample_on_column | 2.0 |
| sample_submission_date | 2018-04-05 |
| sample_type | HLB-cleaned dried peptides |
| serovar | M1T1 clone |
| strain_description | Extensively studied, global reference strain. Representative of serotype M1T1 clone. Tn-seq library available |
| strain_or_isolate | 5448 |
| taxon_or_organism | Streptococcus pyogenes |
| ticket | BPAOPS-665 |
| treatment | 10mM DTT, 20mM IAA, 1:25µg trypsin 37C 24h |