ARP Proteomics SwathMS 51627
Dataset size is: 2.14 GiB
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Additional Info Show Blank Fields
Field | Value |
---|---|
Resource Permissions | organization_member |
analytical_platform | SWATH-MS |
archive_ingestion_date | 2019-01-30 |
contact_researcher | Dick Strugnell |
contextual_data_submission_date | 2018-08-17 |
culture_collection_date | 2002-10-23 |
data_generated | True |
data_type | Main dataset |
facility | APAF |
gradient_time_per_acn | 600nL/min, 2% to 10% B, 10 min, 10% B to 35% B, 78 min |
gram_stain | NEG |
growth_condition_growth_phase | N/A |
growth_condition_media | Pooled sera |
growth_condition_od600_reading | N/A |
growth_condition_temperature | 37.0 |
growth_condition_time_(h) | 2 hrs exposure to sera |
growth_media | Pooled sera |
host_age | 66 |
host_disease_outcome | Sepsis |
host_location | Victoria, Australia (Alfred Hospital, Melbourne) |
host_sex | F |
isolation_source | Blood |
lc_column_type | Eksigent Ultra nanoLC system (Eksigent), Halo C18, 100 mm × 150 µm, 160Å, 2.7 µm |
mass_spectrometer | 6600 Triple TOF |
omics | Proteomics |
peptide_resuspension_protocol | Tip-probe sonicate 2x 30s in solvent intended for injection (e.g 90% MeCN/0.1% TFA) |
replicate | 5 |
sample_id | 102.100.100/51627 |
sample_submission_date | 2018-08-21 |
sample_type | HLB-cleaned peptides |
serovar | K61 |
strain_or_isolate | AJ292 |
taxon_or_organism | Klebsiella variicola |
ticket | BPAOPS-778 |
treatment | 10mM DTT, 20mM IAA, 1:25µg trypsin 37C 24h |