Common Scaly-foot, Phylogenomics, Illumina Capture, liver

Pygopodidae, Pygopus lepidopodus, SAMA ABTC34649, reptile, Project Lead: Ian Brennan

Dataset size is: 0.00 Bit

Log in or Register to access resource
 
 

 

Data and Resources

This data is made available openly under a Creative Commons Attribution license. Please see the initiative Data Policy for attribution information.

Additional Info Show Blank Fields

Field Value
Geospatial Coverage

Dataset extent

Map tiles & Data by OpenStreetMap, under CC BY SA.
Resource Permissions organization_member_after_embargo:date_of_transfer_to_archive:365:ausarg-consortium-members
Access Control Date 2023-07-03
Access Control Mode date
Sequence Data Type Illumina Capture
access_rights No restrictions
ala_specimen_url https://biocache.ala.org.au/occurrences/9b1b3090-20f9-4bc5-840e-a390ec08530d
analysis_software Bcl2Fastq
ancillary_notes NA
associated_media NA
barcode_id unknown
base_url https://downloads-qcif.bioplatforms.com/bpa/ausarg_staging/exon_capture/brf/BPAOPS-1367/20220701_AusARG_BRF_351836_HGJFGDRX2/
certainty NA
class Reptilia
collection_date 1991-11-03
collection_method unknown
collector unknown
common_name Common Scaly-foot
coord_uncertainty_metres unknown
country Australia
data_context Phylogenomics
data_custodian Ian Brennan
data_type Illumina Capture
dataset_id 102.100.100/351836
dataset_url WAS BPAOPS-1297
date_of_transfer 2022-07-03
date_of_transfer_to_archive 2022-07-19
decimal_latitude_public -34.9878
decimal_longitude_public 140.8378
description Short reads
dna_treatment 50 Biorupter cycles
experimental_design Capture probes
facility BRF
facility_project_code NA
family Pygopodidae
file_type FASTQ
flowcell_id HGJFGDRX2
flowcell_type Novaseq SP
folder_name 20220701_AusARG_BRF_351836_HGJFGDRX2
genotypic_sex not determined
genus Pygopus
habitat unknown
identified_by unknown
insert_size_range 150bp PE
institution_name South Australian Museum
latitude -34.9878
library_comments First post library prep PCR didn't produce enough DNA, so second half of libraries were PCR'd with 12 cycles, split across 2 reactions and combined before cleaning.
library_construction_protocol NEXTFLEX_2.0_RapidDNASeq
library_id 102.100.100/354692
library_index_id P7_UDI_0750
library_index_id_dual P5_UDI_0750
library_index_seq CCCACGAATG
library_index_seq_dual TCCTATCTTC
library_layout paired end
library_location ANU EBL Freezer
library_ng_ul 10.2
library_oligo_sequence GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTATCTTCATCTCGTATGCCGTCTTCTGCTTG
library_oligo_sequence_dual AATGATACGGCGACCACCGAGATCTACACCCCACGAATGACACTCTTTCCCTACACGACGCTCTTCCGATCT
library_pcr_cycles 12
library_pcr_reps 2
library_prep_date 2022-05-17
library_prepared_by Liz Broady
library_selection Hybrid Selection
library_source GENOMIC
library_strategy Targeted-Capture
library_type Exon capture
lifestage unknown
location_text 4 KM S OF MOOTATUNGA
longitude 140.8378
material_conc_ng_ul 264.0
material_extracted_by Ian G Brennan
material_extraction_date 2021-07-01
material_extraction_method Salt extraction
material_extraction_type DNA
metadata_revision_date 2023-03-14
metadata_revision_filename AusARG_Metadata_master_QCIF_20230314.xlsx
method_of_determination not determined
n_libraries_pooled 33
order Squamata
phenotypic_sex not determined
phylum Chordata
prior_genetics unknown
project_aim Phylogenomics
sample_custodian Stephen Donnellan
sample_id 102.100.100/410844
sample_quality unknown
sequencing_facility Biomolecular Research Facility - ANU
sequencing_kit_chemistry_version 300 cycles
sequencing_model NovaSeq 6000
sequencing_platform Illumina
source_population NA
species lepidopodus
species_name Pygopus lepidopodus
specimen_id SAMA ABTC34649
specimen_id_description South Australian Museum Australian Biological Tissue Collection
state_or_region South Australia
subspecies NA
taxon_id 207567
taxonomic_group reptile
ticket BPAOPS-1367
tissue_collection South Australian Museum Australian Biological Tissue Collection
tissue_number ABTC34649
tissue_preservation ethanol
tissue_type liver
type_status unknown
voucher_or_tissue_number ABTC34649
wild_captive wild
work_order 13047