Stemcell Metabolomics 102.100.100/29592 LC/MS
Dataset size is: 200.82 MiB
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Additional Info Show Blank Fields
| Field | Value |
|---|---|
| Resource Permissions | organization_member |
| acquisition_mode | Scan (85-1200 m/z; 0.8 spectra/s) |
| analytical_platform | LC/MS |
| archive_ingestion_date | 2017-04-21 |
| bpa_dataset_id | 102.100.100/52064 |
| cell_type | ESC |
| contextual_data_submission_date | 2016-01-11 |
| data_generated | 2017-04-21 |
| data_type | mzML and tar.gz files |
| dataset_url | https://downloads-qcif.bioplatforms.com/bpa/stemcell/raw/metabolomic/ma/BPAOPS-130/ |
| date_of_transfer | 2017-04-21 |
| description | David Elliot lab LC-MS cell samples-Raw |
| disease_state | BMPR2 mutant |
| facility | MA - Bio21 |
| folder_name | 20170421_MA_BPA_SC_20161115_LCMS |
| growth_protocol | EGM-2V (Lonza CC3202) |
| instrument_column_type | Agilent 1200 LC system/SeQuant ZIC-p HILIC column (5µm, 150 x 4.6 mm; Millipore) |
| mass_spectrometer | Agilent Q-TOF mass spectrometer 6545 |
| method | Binary gradient made up of solvent A, 20mM ammonium carbonate solution made up in water, and solvent B, 100% acetonitrile using flow rate of 0.3mL/minute. Solvent B gradient was reduced from 80% to 50% between 0.5 and 15.5 minutes, then reduced to 30% between 15.5 and 17.5 minutes. This gradient was further reduced to 5% between 17.5 and 18.5 minutes, kept at 5% until 23 minutes, increased back up to 80% after 23 minutes and kept at 80% until 29 minutes. |
| omics | Metabolomics |
| replicate_group_id | 1.0 |
| research_group | David Elliot |
| sample_description | p1 Endothelial cells BMPR2 kinase KO |
| sample_fractionation_extraction_solvent | Polar metabolite / Methanol:Water:Chloroform |
| sample_id | 102.100.100/29592 |
| sample_name | 2 |
| sample_submission_date | 2016-01-11 |
| species | Homo sapiens |
| stem_cell_line | PSC - Mesendoderm (cardiac differentation) |
| stem_cell_state | Directed differentation |
| submitter | Elizabeth Ling |
| ticket | BPAOPS-130 |
| total_samples | 25 |