Eastern underground orchid, Reference genome, PromethION, 11 flowers from single inflorescence

Orchidaceae, Rhizanthella slateri, RHIZslat2401, plant, Project Lead: Rod Peakall

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Data and Resources

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Additional Info Show Blank Fields

Field Value
Resource Permissions organization_member_after_embargo:date_of_transfer_to_archive:365:tsi-consortium-members
Access Control Date 2026-07-10
Access Control Mode date
Sequence Data Type ont-promethion
access_rights Location information restricted
ala_specimen_url https://bie.ala.org.au/species/https://id.biodiversity.org.au/taxon/apni/5141299
analysis_software MinKNOW 25.03.7
analysis_software_version ONT long reads
base_url https://downloads-qcif.bioplatforms.com/bpa/tsi_staging/ont-promethion/BPAOPS-1826/20250630_TSI_BRF_PBE66586/
bioplatforms_project Threatened Species Initiative
ccg_jira_ticket BPAOPS-1826
cell_postion 1D
certainty certain
class Magnoliopsida
collection_date 2024-09-27
collection_method picked
collector Rod Peakall
collector_sample_id 24007.0
common_name Eastern underground orchid
country Australia
data_context Reference genome
data_custodian Rod Peakall
data_type PromethION
dataset_id 102.100.100/629207
date_of_transfer 2025-07-10
date_of_transfer_to_archive 2025-07-14
dna_treatment Blue pippin 20 Kb size selection
experimental_design Super-accurate basecalling v4.3.0, 400 bps
facility_project_code ONT_gDNA169_959
facility_sample_id 959.0
family Orchidaceae
file_type pod5 bvz
flowcell_id PBE66586
flowcell_type FLO-PRO114M
folder_name 20250630_TSI_BRF_PBE66586
genus Rhizanthella
habitat Eucalyptus forest
insert_size_range 27000.0
institution_name ANU
library_construction_protocol Ligation prep with SQK-LSK114
library_id 102.100.100/682094
library_layout NA
library_location BRF labs
library_ng_ul 33.2
library_prep_date 2025-06-19
library_prepared_by Carolina Correa-Ospina
library_selection random
library_source genomic
library_strategy WGS
library_type ONT-PromethION
lifestage adult
location_text Bulahdelah (precise details withhold as a condition of permit)
material_conc_ng_ul 222.0
material_extracted_by Ashley Jones
material_extraction_date 2025-06-05
material_extraction_method HMW DNA extracted using the following bead based method: Jones et al (2021) HMW DNA extraction, clean-up and size selection for long-read sequencing. PLOS ONE 16(7): e0253830. https://doi.org/10.1371/journal.pone.0253830
material_extraction_type DNA
metadata_revision_date 2025-07-03
metadata_revision_filename 20250704_Sample_metadata_COMBINED-FOR-PORTAL_NOLATLON.xlsx
movie_length 4d8h
order Asparagales
phenotypic_sex hermaphrodite
phylum Streptophyla
sample_custodian Rod Peakall
sample_id 102.100.100/670090
sample_type Single inflorescence
scientific_name Rhizanthella slateri
scientific_name_authorship (Rupp) M.A.Clem. & P.J.Cribb, 1984
sequencing_facility BRF
sequencing_kit_chemistry_version SQK-LSK114M
sequencing_model ONT PromethION
sequencing_platform Oxford Nanopore
species slateri
specimen_id RHIZslat2401
specimen_id_description Peakall lab unique inflorescence field collection number
state_or_region New South Wales
taxon_id 177053.0
taxonomic_group plant
ticket BPAOPS-1826
tissue_collection Peakall lab field collection
tissue_collection_type Living in wild
tissue_number RHIZslat2401-Flowers-24007
tissue_preservation liquid nitrogen
tissue_preservation_temperature -80.0
tissue_type 11 flowers from single inflorescence
wild_captive wild
work_order 14102.0